Testosterone is the primary androgen hormone produced predominantly by the Leydig cells of the testes in males and, to a lesser extent, by the ovaries and adrenal glands in females. It circulates in the bloodstream both bound to sex hormone-binding globulin (SHBG) and albumin, and as free (unbound) testosterone, with the free fraction being biologically active. Measurement of total testosterone is the standard initial assessment, though free or bioavailable testosterone may be clinically relevant in certain contexts. Testosterone biosynthesis is regulated by the hypothalamic-pituitary-gonadal (HPG) axis, wherein gonadotropin-releasing hormone (GnRH) stimulates pituitary release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which in turn drive gonadal testosterone production. In males, testosterone is essential for spermatogenesis, development and maintenance of secondary sexual characteristics, bone density, muscle mass, erythropoiesis, and libido. In females, testosterone contributes to libido, bone health, muscle function, and overall well-being, though at substantially lower physiological concentrations.
Testosterone is a hormone made mainly in the testes in men and in smaller amounts in the ovaries and adrenal glands in women. It plays important roles in sexual development, muscle and bone strength, energy levels, and mood. Levels that are too high or too low can cause a variety of symptoms and may signal an underlying health condition. A blood test can measure your testosterone level, and your doctor will interpret the result alongside your symptoms and other test findings. It is important not to interpret this result in isolation, as many factors can influence testosterone levels.
When elevated: Elevated testosterone in males may suggest exogenous androgen use, androgen-secreting tumors, or congenital adrenal hyperplasia. In females, elevated testosterone is associated with polycystic ovary syndrome (PCOS), adrenal or ovarian androgen-secreting tumors, and congenital adrenal hyperplasia, and may manifest as hirsutism, acne, menstrual irregularity, and virilization. When low: Low testosterone in males (hypogonadism) is associated with decreased libido, erectile dysfunction, fatigue, reduced muscle mass, increased adiposity, osteoporosis, mood disturbances, and impaired fertility. In females, low testosterone may contribute to reduced libido, fatigue, and decreased bone density, though clinical thresholds are less well established.
Testosterone supports muscle protein synthesis, strength gains, bone density, and recovery—key factors in training adaptation and performance. Athletes may track testosterone to monitor training stress, recovery adequacy, and potential overtraining; however, single measurements have limited predictive value without trend data and clinical correlation.
Turnaround Time
3 days (up to 4 days)
Fasting Required
No
Method
Electrochemiluminescence immunoassay (ECLIA)
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$9.39
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