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Biomarkervitamins minerals

Vit. D, 25-Hydroxy Tot.,D2+D3

25-Hydroxyvitamin D [25(OH)D] is the major circulating form of vitamin D and the accepted clinical marker for assessing overall vitamin D status, reflecting contributions from both dietary ergocalciferol (D2) and cholecalciferol (D3) as well as cutaneous synthesis. It is produced primarily in the liver via hydroxylation of vitamin D precursors and serves as the substrate for renal conversion to the biologically active hormone 1,25-dihydroxyvitamin D (calcitriol). Total 25(OH)D measurement captures both D2 and D3 metabolites, providing a comprehensive assessment of vitamin D stores. Vitamin D undergoes two sequential hydroxylation steps: first in the liver to form 25(OH)D, then in the kidneys (and other tissues) to form 1,25(OH)₂D (calcitriol), which binds the vitamin D receptor (VDR) to regulate calcium and phosphate homeostasis, parathyroid hormone secretion, and numerous genomic pathways. The half-life of 25(OH)D is approximately 2–3 weeks, making it a reliable indicator of medium-term vitamin D status rather than acute fluctuations. Beyond skeletal health, vitamin D signaling influences immune modulation, cell differentiation, and neuromuscular function through widespread VDR expression across body tissues.

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What this test reveals

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This test measures the total amount of vitamin D circulating in your blood, combining two forms (D2 and D3) to give an overall picture of your vitamin D stores. Vitamin D is essential for strong bones, muscle function, and a healthy immune system, and your body makes it when your skin is exposed to sunlight. Low levels are common, especially in people who spend little time outdoors, have darker skin, or have certain digestive conditions. Very high levels—usually from taking too many supplements—can also cause health problems. Your healthcare provider will consider your individual health history when interpreting this result.

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What abnormal values may indicate

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When elevated: Elevated 25(OH)D levels (typically >100–150 ng/mL) may indicate vitamin D toxicity (hypervitaminosis D), which can lead to hypercalcemia, hypercalciuria, nephrolithiasis, soft tissue calcification, and renal impairment. Mild-to-moderate elevations above the reference range warrant clinical correlation and review of supplementation practices. When low: Low 25(OH)D is associated with impaired calcium absorption, secondary hyperparathyroidism, reduced bone mineral density, increased fracture risk, muscle weakness, and in severe cases, rickets (children) or osteomalacia (adults). Observational data also link deficiency to increased susceptibility to infections and adverse musculoskeletal outcomes.

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For athletes

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Vitamin D supports calcium regulation, muscle function, and immune health—all important for training recovery and injury prevention. Athletes with limited sun exposure or those training indoors may benefit from monitoring this marker, though vitamin D's direct performance impact is modest compared to other factors like training load and nutrition.

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Turnaround Time

3 days (up to 7 days)

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Fasting Required

No

scienceExpected Results

3 results
25-OHVitamin D Total25-OH Vitamin D225-OH Vitamin D3
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How it works

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    Choose your lab and check out. We send your lab requisition automatically — no doctor visit needed.

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    Get your sample collected

    Visit a lab service center near you for a quick blood draw (or book at-home phlebotomy where available).

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    See your results

    Your results land in your Insider portal, ready to review and act on — that easy.

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Your price

$46.90$140

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3.0× less than retail

Versus the typical direct-to-consumer retail price for this test (illustrative — consumer prices vary by provider and region).

BioReference

$46.90

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References (3)

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